meatmilkАктуальные вопросы переработки мясного и молочного сырьяTopical Issues of Processing of Meat and Milk Raw Materials2220-8755Институт системных исследований в АПК Национальной академии наук Беларусиmeatmilk-144Research ArticleСтатьиМОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКАЯ ДИФФЕРЕНЦИАЦИЯ БАКТЕРИЙ РОДА LACTOCOCCUSMOLECULAR-GENETIC DIFFERENTIATION OF LACTOCOCCUSБирюкЕ. Н.BirukA. N.

Минск

СысолятинЕ. Н.SysaliatsinE. N.

Минск

ЯцевичК. К.YatsevichK. K.

Минск

ГалиновскийД. В.GalinouskyD. V.

Минск

ФурикН. Н.FurikN. N.

Минск

Институт мясо-молочной промышленностиBelarusИнститут генетики и цитологии НАН БеларусиBelarus20130605202108157169Copyright © Бирюк Е.Н., Сысолятин Е.Н., Яцевич К.К., Галиновский Д.В., Фурик Н.Н., 20212021Бирюк Е.Н., Сысолятин Е.Н., Яцевич К.К., Галиновский Д.В., Фурик Н.Н.Biruk A.N., Sysaliatsin E.N., Yatsevich K.K., Galinousky D.V., Furik N.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://meatmilk.belal.by/jour/article/view/144

В работе проведена оценка методов внутривидовой генотипической дифференциации бактерий вида Lactococcus lactis. Установлено, что наиболее эффективны методы Rep- и RAPD-ПЦР. Метод риботипирования (ITS-RFLP) оказался непригодным для внутривидовой дифференциации бактерий данного вида. В результате генотипирования коллекционные штаммы бактерий (20 штаммов) были разделены на четыре генетически гетерогенные группы.

Lactococcus lactis subspecies differentiations methods were examined. It was received that the most effective differential methods are Rep- and RAPD-PCR. The ribotyping method is not suitable to distinguish the subspecies of Lactococcus lactis. According the genоtyping analysis twenty collection bacteria strains were divided into four groups which were genetic heterogeneous.

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The authors declare that there are no conflicts of interest present.