meatmilkАктуальные вопросы переработки мясного и молочного сырьяTopical Issues of Processing of Meat and Milk Raw Materials2220-8755Институт системных исследований в АПК Национальной академии наук Беларусиmeatmilk-426Research ArticleБИОТЕХНОЛОГИЯСиквенс-типирование Lactobacillus helveticus и Lactobacillus acidophillus на основании 3 генов домашнего хозяйстваSequence typing of Lactobacillus helveticus and Lactobacillus acidophillus based on 3 housekeeping genesШукшинаМ. А.ShukshynaM. A.

Минск

Minsk

shucshina.margarita@yandex.byБирюкЕ. Н.BirukE. N.

к.с-х.н.,

Минск

Minsk

biohimbel@rambler.ruРадиончикМ. О.RadzivonchykM. O.

Минск

Minsk

martaclarke93@gmail.comИнститут мясо-молочной промышленностиInstitute for Meat and Dairy Industry202227022024017107114Copyright © Шукшина М.А., Бирюк Е.Н., Радиончик М.О., 20242024Шукшина М.А., Бирюк Е.Н., Радиончик М.О.Shukshyna M.A., Biruk E.N., Radzivonchyk M.O.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://meatmilk.belal.by/jour/article/view/426

В статье представлены результаты исследований сиквенс-типирования штаммов Lactobacillus helveticus и Lactobacillus acidophilus с использованием 3 пар праймеров (TestGB, Sgro и SrplB), сконструированых к генам «домашнего хозяйства» gyrB, groEL и rplB, которые позволяют достоверно идентифицировать бактерии Lactobacillus helveticus и Lactobacillus acidophilus и типировать штаммы внутри видов.

This article presents the results of research on sequence-typing of Lactobacillus helveticus and Lactobacillus acidophilus using 3 primer sets (TestGB, Sgro, SrplB), designed based on the housekeeping genes gyrB, groEL and rplB, which allow reliable identification of Lactobacillus helveticus and Lactobacillus acidophilus and typing of strains within species.

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The authors declare that there are no conflicts of interest present.