д.б.н.
Москва
Moscow
д.м.н.
Москва
Moscow
к.б.н.
Москва
Moscow
Москва
Moscow
Москва
Moscow
Москва
Moscow
Москва
Moscow
Москва
Moscow
Представлены результаты первого этапа работ по гранту Российского научного фонда №23-16-00163 «Эмерджентные патогены рода Cronobacter (E. sakazakii) в продуктах для питания детей отечественного производства: новые аспекты безопасности». Установлено присутствие С.sakazakii в сухих продуктах для вскармливания и прикорма детей раннего возраста, а также компонентах для их изготовления, циркулирующих на рынке РФ, с частотой от 1,6% до 11,1%% при уровнях патогена 0,01 - 10 КОЕ/г, опасных для восприимчивого организма. Наибольшая частота выявления отмечена в компонентах, предназначенных для сухого смешивания. Оценка технологии молочных смесей полного цикла по методологии оценки микробиологического риска показала возможность сохранения патогена в количествах, превышающих установленный норматив для C.sakazakii в готовой продукции (<0,003 KOE/г), при содержании БГКП в сыром молоке 3х105 КОЕ/г и выше. Обоснована необходимость повышения требований к микробиологическому качеству молока сырого для производства детского питания, а также внедрения в контроль патогенов рода Cronobacter в продуктах для детей раннего возраста методов молекулярного анализа. Для снижения риска загрязнения Cronobacter spp. окружающей среды на предприятиях, вырабатывающих сухие детские продукты, обоснована необходимость оптимизации систем НАССР на всех этапах производства, включая дезинфекцию оборудования и инвентаря. Это связано с подтверждённой большей устойчивостью C.sakazakii к воздействию температур, дезинфектантов в сравнении с другими видами контаминирующих сухие продукты энтеробактерий.
The results of the first stage of work under the grant of the Russian Science Foundation No. 23-16-00163 "Emergent pathogens of the genus Cronobacter (E. sakazakii) in domestically produced baby food products: new safety aspects" are presented.The presence of C.sakazakii in dry products for feeding and complementary feeding of young children, as well as components for their manufacture, circulating on the Russian market, was established with frequency from 1.6% to 11.1%% at levels 0,01 - 10 CFU/g, dangerous for a susceptible organism. The highest frequency of pathogen detection was recorded in components intended for dry mixing. The results of the first stage of work under the grant of the Russian Science Foundation No. 23-16-00163 "Emergent pathogens of the genus Cronobacter (E. sakazakii) in domestically produced baby food products: new safety aspects" are presented.The presence of C.sakazakii in dry products for feeding and complementary feeding of young children, as well as components for their manufacture, circulating on the Russian market, was established with frequency from 1.6% to 11.1%% at levels 0,01 - 10 CFU/g, dangerous for a susceptible organism. The highest frequency of pathogen detection was recorded in components intended for dry mixing.The presence of C.sakazakii in dry products for feeding and complementary feeding of young children, as well as components for their manufacture, circulating on the Russian market, was established with frequency from 1.6% to 11.1%% at levels 0,01 - 10 CFU/g, dangerous for a susceptible organism. The highest frequency of pathogen detection was recorded in components intended for dry mixing. Assessment of the full-cycle milk formulas technology using the microbiological risk assessment methodology showed the possibility of preserving of pathogen in quantities exceeding the established standard for C.sakazakii in finished products (<0.003 CFU/g), with a coliforms content in raw milk of 3x105 CFU/g and higher.The need to increase the requirements for the microbiological quality of raw milk for the production of baby food, as well as the introduction of molecular analysis methods for the control of pathogens of the genus Cronobacter in products for young children, is substantiated.To reduce the risk of Cronobacter spp. contamination of the environment at enterprises producing dry infants foods, the need to optimize HACCP systems at all stages of production, including disinfection of equipment and inventory is substantiated. This is due to the confirmed greater resistance of C.sakazakii to the effects of temperatures, disinfectants in comparison with other types of enterobacteria contaminating dry products.
The authors declare that there are no conflicts of interest present.