STUDY OF PROTECTIVE MEDIA INFLUENCE ON MICROORGANISMS KEEPING IN CONCENTRATED BIFIDOBACTERIUM LONGUM BIOMASS IN THE PROCESS OF CRYOPELLETIZING AND STORAGE
Abstract
The article presents the results of studies about of the optimal composition of the protective medium for cryoconservation of Bifidobacterium longum 432 OR concentrated biomass. We froze concentrated biomass in liquid nitrogen and studied its survival in storage process. As a result of the research the cryoprotective medium was constructed. This medium contained sucrose, lactose, sodium citrate, skimmed milk with 0.03% sodium citrate and inulin and provided maximum preservation of starter culture during cryoconservation and further storage.
About the Authors
V. Taras
Institute for Meat and Dairy Industry
Belarus
Belarus
Minsk
N. Zhabanos
Institute for Meat and Dairy Industry
Belarus
Belarus
Minsk
N. Furyk
Institute for Meat and Dairy Industry
Belarus
Belarus
Minsk
References
1. Shramko, M.I. Nauchno-prakticheskoe obosnovanija novyh bakterial'nyh koncentratov s kriozamorazhivaniem mikrobnoj biomassy dlja poluchenija kormovyh dobavok probioticheskogo dejstvija na osnove vtorichnogo molochnogo syr'ja pri vyrashhivanii teljat-molochnikov [The scientific and practical justification of new concentrated starter cultures with cryofreezing of microbial biomass for receiving feed additives of pro-biotic action on the basis of secondary lactic raw materials at breeding of calfs]: diss. … k.b.n.: 06.02.10/ M.I. Shramko; Povolzhsk. nauch.-issled.in-t proizvodstvai pererabotki mjasomolochnoj produkcii RASHN. – Volgograd, 2011. – 139 l.
2. Semenihina, V.F. Osobennosti ispol'zovanija bifidobakterij pri proizvodstve probioticheskih kislomolochnyh produktov [The features of use of bifidobacteria by production of pro-biotic fermented milk products] / V.F. Semenihina, I.V. Rozhkova, A.V. Begunova // Nauka proizvodstvu: inf. Bjulleten' / GNU VNIMI Rossel'hozakademii. – M., 2011.– №2. – S.15–17.
3. Tumunova, S.B. Razrabotka tehnologii proizvodstva suhogo koncentrata bifidobakterij [Development of the production technology of the freeze-dried concentrated starter culture of bifidobacteria] avtoref. dis. … kand. tehn. nauk: 637.146.33 / S.B. Tumunova; GNU VSGTU RASHN. – Ulan-Ude, 1995. – 20 s.
4. Hamagaeva, I.S. Nauchnye osnovy biotehnologii kislomolochnyh produktov dlja detskogo i dieticheskogo pitanija [Scientific bases of biotechnology of fermented milk products for a children's and dietary food]: monografija/ I.S. Hamagaeva. – Ulan-Udje: Izd-vo VSGTU, 2005. – 279 s.
5. Krus', G.N. Tehnologija moloka i molochnyh produktov [Technology of milk and dairy products] / G.N. Krus', A.G. Hramcov, Z.V. Volokitina, S.V. Karpychev; pod red. A.M. Shalyginoj. – M: Kolos, 2004. – 455 s.
6. Cucaeva, A.A. Opyt dolgosrochnogo hranenija promyshlennyh shtammov mikroorganizmov [Experience of long-term storage of the industrial strains of microorganisms] / A,A. Cucaeva, A.E. Anan'ina, L.M. Balyberdina, L.V. Stepanjuk, N.V. Pavlenko // Mikrobiologija. – 2008. – T.77, №5. – S.696–700.
7. Romanova, Je.V. Harakteristika shtammov laktobakterij [Characteristic of strains Lactobacillus] / Je.V. Romanova, R.G. Kabisov, B.G. Cugkiev // Molochnaja promyshlennost'. – 2009. – № 2. – S. 43.
8. Virnik, A.D. Dekstran i ego proizvodnye [Dextran and its derivants] / A.D. Virnik, K.P. Homjakov, I.F. Skokova // Uspehi himii. – 1975. – № 44. – S. 1280.
9. Artjuhov, I.V. Kriosohranenie krupnyh biologicheskih ob'ektov [Cryopreservation of large biological objects] [Jelektronnyj resurs] / I.V. Artjuhov, A.V. Karnauhov // Zhurnal «Biologija». – 2003. – №43 Rezhim dostupa: http://bio.1september.ru/view_article.php?ID=200304301. – Data dostupa: 30.03.2017.
10. Fernandez Murga, M. L. Influence of growth temperature on cryotolerance and lipid composition of Lactobacillus acidophilus // M.L. Fernandez Murga, G.M. Cabrega, G.F. De Valdez, A. Disalvo, A.M. Seldes // Journal of applied microbiology. – 2000. – Vol. 88, №2. – P. 342–348.
11. Cucaeva, A.A. Kriobiologija i biotehnologija [Cryobiology and biotechnology] / A.A.Cucaeva [i dr.] – Kiev: Navukova dumka, 1987. – 214 s.
12. Hubalec, Z. Protectants used in the cryopreservation of microorganisms / Z. Hubalec // Cryobiology. – 2003. – Vol. 46, №3. – P. 205–229.
13. Tamim, A.I. Jogurty i analogichnye kislomolochnye produkty: nauchnye osnovy i tehnologii [Yogurts and similar fermented milk products: scientific bases and technologies] / A.I. Tamim, R.K. Robinson – SPb: Professija, 2003. – 664 s.
14. Kuz'mina, O.M. Issledovanie vlijanija sostava zashhitnoj sredy na jeffektivnost' processa kriozamorazhivanija mikroorganizmov [The research of influence of structure of a protective medium on effectiveness of process of cryofreezing of microorganisms]: diss. … k.t.n.: 05.18.04 / O.M.Kuz'mina. – Moskva. 2010. – 128 s.
15. Tehnologicheskaja instrukcija po izgotovleniju zakvaski zamorozhennoj koncentrirovannoj bifidobakterij. Tehnologicheskaja instrukcija [The technological instruction for manufacture of the the frozen concentrated starter culture bifidobacteria. The technological instruction]: TI BY 100098867.446 – 2016.: Vved. 03.11.2016 (vvedena vpervye). – Minsk: RUP «Institut mjaso-molochnoj promyshlennosti», 2016. – 51 s.
Review
For citations:
Taras V., Zhabanos N., Furyk N. STUDY OF PROTECTIVE MEDIA INFLUENCE ON MICROORGANISMS KEEPING IN CONCENTRATED BIFIDOBACTERIUM LONGUM BIOMASS IN THE PROCESS OF CRYOPELLETIZING AND STORAGE. Topical Issues of Processing of Meat and Milk Raw Materials. 2016;(11):52-58. (In Russ.)
Views: 5537
Email this article 