The isolation of whey β-lactoglobulin
Abstract
The analysis of existing methods for the isolationofwhey β- lactoglobulin is conducted and it is established that the following methods such aschromatography, membrane filtration, precipitation and thermaldenaturation are used actually. The possibility of the isolationofβ- lactoglobulinwith precipitation and thermal denaturation methods is determined in laboratory conditions. Based on the results of the experiments it is established that indicatedmethods can be used to isolate whey β- lactoglobulin, though it is necessary to consider them in the context of possible combination with others.
About the Authors
O. Dymar
Институт мясо-молочной промышленности
Belarus
K. Shehidzevich
Институт мясо-молочной промышленности
Belarus
References
1. Gorbatova, K.K. Khimiyaifizikabelkovmoloka / K.K. Gorbatova. - M.: Kolos, 1993. - 192 s.
2. Whey processing, functionality and health benefits / Institute of Food Technologists;editors: Charles Onwulata, Peter Huth. - Ames, 2008. - 400 r.
3. Sova, V.V. Vydelenie i ochistka belkov. Metodicheskoe posobie po kursu «Khimiya i biokhimiya belkov i fermentov» / V.V. Sova, M.I. Kusaikin. - Vladivostok: Izd-vo Dal'nevost. un-ta, 2006. - 42 s.
4. Advanced Dairy Chemistry. Volume 1A: Proteins: Basic Aspects / editors: Paul L.H. McSweeney, Patrick F. Fox. - New York, 2013. - 548 r.
5. Osterman, L.A. Khromatografiya belkov i nukleinovykh kislot / L.A. Osterman -M.: Nauka, 1985. - 536 s.
6. Fuquay, J.W. Encyclopedia of dairy sciences / J.W. Fuquay, P.F. Fox, P.L.H. McSweeney. - Elsevier Ltd., 2011. - 4068 s.
7. Konrad, G. A large-scale isolation of native β-lactoglobulin: characterization of physicochemical properties and comparison with other methods / G. Konrad, B. Lieske // International Dairy Journal. - 2000. - Vol. 10. - R. 713-721.
For citations:
Dymar O.,
Shehidzevich K.
The isolation of whey β-lactoglobulin. Topical Issues of Processing of Meat and Milk Raw Materials. 2014;1(9):69-76.
(In Russ.)
Views:
209