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PHENOTYPIC AND GENETIC CHARACTERIZATION OF LACTO-COCCAL PHAGES C2 FOR WORKING OUT METHOD OF THEIR GENOTYPING

Abstract

Eight lactococcal phages c2 were characterized by their resistance to physical and chemical factors and lytic spectra. It was found that phenotypic variability of the phages c2 was wide, thus molecular techniques seemed to be essential for their typing. It was demonstrated that intraspecies differentiation of c2 phages is possible by RFLP analysis of mcp gene fragment, amplified by PCR with species-specific primers.

About the Authors

N. N. Furik
Institute for the Meat and Dairy Industry
Belarus


D. P. Bazhanov
Institute of Genetics and Cytology of NAS of Belarus
Belarus


K. K. Yatsevich
Institute of Genetics and Cytology of NAS of Belarus
Belarus


E. A. Kasperovich
Institute for the Meat and Dairy Industry
Belarus


S. L. Vasilenko
Institute for the Meat and Dairy Industry
Belarus


G. V. Mashkovskaya
Institute of Genetics and Cytology of NAS of Belarus
Belarus


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Review

For citations:


Furik N.N., Bazhanov D.P., Yatsevich K.K., Kasperovich E.A., Vasilenko S.L., Mashkovskaya G.V. PHENOTYPIC AND GENETIC CHARACTERIZATION OF LACTO-COCCAL PHAGES C2 FOR WORKING OUT METHOD OF THEIR GENOTYPING. Topical Issues of Processing of Meat and Milk Raw Materials. 2010;(5):152-164. (In Russ.)

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