Sequence typing of Lactobacillus helveticus and Lactobacillus acidophillus based on 3 housekeeping genes
Abstract
This article presents the results of research on sequence-typing of Lactobacillus helveticus and Lactobacillus acidophilus using 3 primer sets (TestGB, Sgro, SrplB), designed based on the housekeeping genes gyrB, groEL and rplB, which allow reliable identification of Lactobacillus helveticus and Lactobacillus acidophilus and typing of strains within species.
About the Authors
M. A. Shukshyna
Institute for Meat and Dairy Industry
Belarus
Belarus
Minsk
E. N. Biruk
Institute for Meat and Dairy Industry
Belarus
Belarus
Minsk
M. O. Radzivonchyk
Institute for Meat and Dairy Industry
Belarus
Belarus
Minsk
References
1. Stefanovic, E. Advances in the genomics and metabolomics of dairy lactobacilli: A review / E. Stefanovic, G. Fitzgerald, O. McAuliffe // Food Microbiol., 2017. – Vol. 61 – P. 33-49.
2. Felis, G. E. Taxonomy of Lactobacilli and Bifidobacteria / G. E. Felis, F. Dellaglio // Curr Issues Intest Microbio. – l2007. – Vol. 8, № 2. – P. 44-61.
3. Accuracy of species identity of commercial bacterial cultures intended for probiotic or nutritional use / G. Huys [et al.] // Research in Microbiology. – 2006. – Vol. 157, № 9. – P. 803-810.
4. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae / J. Zheng [et al.] // Int J Syst Evol Microbiol. – 2020. – Vol. 70, № 4. – P. 2782-2858.
5. Molecular diversity within Lactobacillus helveticus as revealed by genotypic characterization / G. Giraffa [et al.] // Appl. Environ. Microbiol. – 2000. – Vol. 66, № 4. – P. 1259-1265.
6. Gatti, M. Effectiveness of chemometric techniques in discrimination of Lactobacillus helveticus biotypes from natural dairy starter cultures on the basis of phenotypic characteristics / M. Gatti, G. Contarini, E. Neviani // Appl Environ Microbiol. – 1999. – Vol. 65, № 4. – P. 1450-1454.
7. 16S ribosomal DNA amplification for phylogenetic study / W.G. Weisburg [et al.] // J Bacteriol. – 1991. – Vol. 173, № 2. – P. 697-703.
8. Development of a Tiered Multilocus Sequence Typing Scheme for Members of the Lactobacillus acidophilus Complex / P. Ramachandran [et al.] // Appl. Environ. Microbiol. – 2013. – Vol. 79, № 23. – P. 7220-7228.
9. Multilocus sequence typing: A portable approach to the identification of clones within populations of pathogenic microorganisms / M. C. J. Maiden [et al.] // Proceedings of the National Academy of Sciences. – 1998. – Vol. 95, № 6. – P. 3140-3145.
10. Lifestyles in transition: evolution and natural history of the genus Lactobacillus / R. M. Duar [et al.] // FEMS Microbiology Reviews. – 2017. – Vol. 41, № Supp_1. – P. S27-S48.
11. Goldstein, E. J. C. Lactobacillus Species: Taxonomic Complexity and Controversial Susceptibilities / E. J. C. Goldstein, K. L. Tyrrell, D.M. Citron // Clinical Infectious Diseases2. – 015. – Vol. 60, № suppl_2. – P. S98-S107.
12. The domestication of the probiotic bacterium Lactobacillus acidophilus / M. J. Bull [et al.] // Sci Rep, 2014. – Vol. 4 – P. 7202. 13. Genomic Comparison of Lactobacillus helveticus Strains Highlights Probiotic Potential / A. Fontana [et al.] // Front. Microbiol, 2019. – Vol. 10 – P. 1380.
Review
For citations:
Shukshyna M.A., Biruk E.N., Radzivonchyk M.O. Sequence typing of Lactobacillus helveticus and Lactobacillus acidophillus based on 3 housekeeping genes. Topical Issues of Processing of Meat and Milk Raw Materials. 2022;(17):107-114. (In Russ.)
Views: 82
Email this article 